The rapid detection of PCR-SSCP analysis using fluorescently labeled primers.
نویسندگان
چکیده
منابع مشابه
Translation toeprinting assays using fluorescently labeled primers and capillary electrophoresis.
The protocol described here is an adapted version of the toeprinting assay in which the oligonucleotide used to prime the reverse transcription step is labeled with a fluorescent dye instead of 32P. By using a fluorescent dye, the results of the assay are obtained within one hour by direct electrophoresis of the samples on an automated sequencing machine. This eliminates the need to cast and ru...
متن کاملDetection of clonal T cell populations by high resolution PCR using fluorescently labelled nucleotides; evaluation using conventional LIS-SSCP.
AIMS To detect clonal T cell populations by high resolution polymerase chain reaction (PCR) using fluorescently labelled nucleotides and analysis on an ABI 377 DNA sequencer, and to evaluate this method using low ionic strength single strand conformation polymorphism (LIS-SSCP) analysis. METHODS DNA samples from 11 patients diagnosed with a T cell disease and 15 with no known T cell disorder ...
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SSCP analysis is one of the most commonly used techniques for screening of small genetic alterations and a very convenient alternative to direct sequencing. In this technique, PCR products are denatured and electrophoresed on non-denaturing polyacrylamide gels. Any change in the sequence theoretically causes a shift in the mobility of the analysed conformers. However, it is known (1) that certa...
متن کاملRapid identification of Phytophthora ramorum using PCR-SSCP analysis of ribosomal DNA ITS-1.
AIMS The primary objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis can be used for rapid identification of Phytophthora ramorum, an important quarantine plant pathogen worldwide, and to further assess the potential of the SSCP technique as a taxonomic tool for the genus Phytophthora. METHODS AND RESULTS SSCP of ribosomal DNA internal trans...
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We show that a post-PCR multicolor fluorescence-labeling technique is applicable to multiplex microsatellite genotyping. Forty-three dinucleotide microsatellite markers, which are located on 11q13-23, a candidate region for dominant familial exudative vitreoretinopathy (FEVR), were used to evaluate the quality of the marker profile produced by this technique. Thirty-eight people from six famili...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1999
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.43.251